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mgi dnbseq t1 sequencer  (Complete Genomics Inc)


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    Structured Review

    Complete Genomics Inc mgi dnbseq t1 sequencer
    Mgi Dnbseq T1 Sequencer, supplied by Complete Genomics Inc, used in various techniques. Bioz Stars score: 99/100, based on 102 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mgi+dnbseq+t1+sequencer/DNBSEQ-T1%2B/pm41735337-47-7-7
    Average 99 stars, based on 102 article reviews
    mgi dnbseq t1 sequencer - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Purification:

    Article Title: An organ-wide spatiotemporal transcriptomic and cellular atlas of the regenerating zebrafish heart
    Article Snippet: Sequencing libraries were prepared with PCR products undergoing the following steps: concentration quantification (QubitTM dsDNA Assay Kit, Thermo, Q32854), DNA fragmentation (in-house Tn5 transposase at 55 °C for 10 minutes), PCR amplification (KAPA HiFi Hotstart Ready Mix (Roche, KK2602) with 0.3 μM Stereo-seq-Library-F primer (/5phos/CTGCTGACGTACTGAGAGG*C*A) and 0.3 μM Stereo-seq-Library-R primer (GAGACGTTCTCGACTCAGCAGA) and purification (Vazyme, N411-03). .. The purified PCR products were used to construct DNB libraries and sequenced on an MGI DNBSEQ-T1 sequencer (35 bp for read 1, 100 bp for read 2). ..

    Article Title: 4D Single-Cell Spatial Transcriptomics Reveals Dynamic Morphogenetic Gradients and Regenerative Domains in Planarians
    Article Snippet: PCR products were used to prepare sequencing libraries, with the following steps: quantification of concentration using the QubitTM dsDNA Assay Kit (Thermo, Q32854), DNA fragmentation with in-house Tn5 transposase at 55 °C for 10 minutes, PCR amplification (KAPA HiFi Hotstart Ready Mix, Roche, KK2602) with 0.8 μM cDNA-PCR primers, and purification using Vazyme (N411-03). .. The purified PCR products were used to construct DNB libraries and sequenced on an MGI DNBSEQ-T1 sequencer (35 bp for Read1, 100 bp for Read2). ..

    Article Title: An organ-wide spatiotemporal transcriptomic and cellular atlas of the regenerating zebrafish heart.
    Article Snippet: Sequencing libraries were prepared with PCR products undergoing the following steps: concentration quantification (QubitTM dsDNA Assay Kit, Thermo, Q32854), DNA fragmentation (in-house Tn5 transposase at 55 °C for 10minutes), PCR amplification (KAPA HiFi Hotstart ReadyMix (Roche, KK2602) with 0.3μM Stereo-seq-Library-F primer (/5phos/ CTGCTGACGTACTGAGAGG*C*A) and 0.3μM Stereo-seq-Library-R primer (GAGACGTTCTCGACTCAGCAGA) and purification (Vazyme, N411-03). .. The purified PCR products were used to construct DNB libraries and sequenced on an MGI DNBSEQ-T1 sequencer (35 bp for read 1, 100 bp for read 2). ..

    Polymerase Chain Reaction:

    Article Title: An organ-wide spatiotemporal transcriptomic and cellular atlas of the regenerating zebrafish heart
    Article Snippet: Sequencing libraries were prepared with PCR products undergoing the following steps: concentration quantification (QubitTM dsDNA Assay Kit, Thermo, Q32854), DNA fragmentation (in-house Tn5 transposase at 55 °C for 10 minutes), PCR amplification (KAPA HiFi Hotstart Ready Mix (Roche, KK2602) with 0.3 μM Stereo-seq-Library-F primer (/5phos/CTGCTGACGTACTGAGAGG*C*A) and 0.3 μM Stereo-seq-Library-R primer (GAGACGTTCTCGACTCAGCAGA) and purification (Vazyme, N411-03). .. The purified PCR products were used to construct DNB libraries and sequenced on an MGI DNBSEQ-T1 sequencer (35 bp for read 1, 100 bp for read 2). ..

    Article Title: 4D Single-Cell Spatial Transcriptomics Reveals Dynamic Morphogenetic Gradients and Regenerative Domains in Planarians
    Article Snippet: PCR products were used to prepare sequencing libraries, with the following steps: quantification of concentration using the QubitTM dsDNA Assay Kit (Thermo, Q32854), DNA fragmentation with in-house Tn5 transposase at 55 °C for 10 minutes, PCR amplification (KAPA HiFi Hotstart Ready Mix, Roche, KK2602) with 0.8 μM cDNA-PCR primers, and purification using Vazyme (N411-03). .. The purified PCR products were used to construct DNB libraries and sequenced on an MGI DNBSEQ-T1 sequencer (35 bp for Read1, 100 bp for Read2). ..

    Article Title: An organ-wide spatiotemporal transcriptomic and cellular atlas of the regenerating zebrafish heart.
    Article Snippet: Sequencing libraries were prepared with PCR products undergoing the following steps: concentration quantification (QubitTM dsDNA Assay Kit, Thermo, Q32854), DNA fragmentation (in-house Tn5 transposase at 55 °C for 10minutes), PCR amplification (KAPA HiFi Hotstart ReadyMix (Roche, KK2602) with 0.3μM Stereo-seq-Library-F primer (/5phos/ CTGCTGACGTACTGAGAGG*C*A) and 0.3μM Stereo-seq-Library-R primer (GAGACGTTCTCGACTCAGCAGA) and purification (Vazyme, N411-03). .. The purified PCR products were used to construct DNB libraries and sequenced on an MGI DNBSEQ-T1 sequencer (35 bp for read 1, 100 bp for read 2). ..

    Construct:

    Article Title: An organ-wide spatiotemporal transcriptomic and cellular atlas of the regenerating zebrafish heart
    Article Snippet: Sequencing libraries were prepared with PCR products undergoing the following steps: concentration quantification (QubitTM dsDNA Assay Kit, Thermo, Q32854), DNA fragmentation (in-house Tn5 transposase at 55 °C for 10 minutes), PCR amplification (KAPA HiFi Hotstart Ready Mix (Roche, KK2602) with 0.3 μM Stereo-seq-Library-F primer (/5phos/CTGCTGACGTACTGAGAGG*C*A) and 0.3 μM Stereo-seq-Library-R primer (GAGACGTTCTCGACTCAGCAGA) and purification (Vazyme, N411-03). .. The purified PCR products were used to construct DNB libraries and sequenced on an MGI DNBSEQ-T1 sequencer (35 bp for read 1, 100 bp for read 2). ..

    Article Title: 4D Single-Cell Spatial Transcriptomics Reveals Dynamic Morphogenetic Gradients and Regenerative Domains in Planarians
    Article Snippet: PCR products were used to prepare sequencing libraries, with the following steps: quantification of concentration using the QubitTM dsDNA Assay Kit (Thermo, Q32854), DNA fragmentation with in-house Tn5 transposase at 55 °C for 10 minutes, PCR amplification (KAPA HiFi Hotstart Ready Mix, Roche, KK2602) with 0.8 μM cDNA-PCR primers, and purification using Vazyme (N411-03). .. The purified PCR products were used to construct DNB libraries and sequenced on an MGI DNBSEQ-T1 sequencer (35 bp for Read1, 100 bp for Read2). ..

    Article Title: An organ-wide spatiotemporal transcriptomic and cellular atlas of the regenerating zebrafish heart.
    Article Snippet: Sequencing libraries were prepared with PCR products undergoing the following steps: concentration quantification (QubitTM dsDNA Assay Kit, Thermo, Q32854), DNA fragmentation (in-house Tn5 transposase at 55 °C for 10minutes), PCR amplification (KAPA HiFi Hotstart ReadyMix (Roche, KK2602) with 0.3μM Stereo-seq-Library-F primer (/5phos/ CTGCTGACGTACTGAGAGG*C*A) and 0.3μM Stereo-seq-Library-R primer (GAGACGTTCTCGACTCAGCAGA) and purification (Vazyme, N411-03). .. The purified PCR products were used to construct DNB libraries and sequenced on an MGI DNBSEQ-T1 sequencer (35 bp for read 1, 100 bp for read 2). ..

    Sequencing:

    Article Title: A spatial transcriptomics comparison of the adult versus metamorphosed axolotl brain
    Article Snippet: .. Raw sequencing data were generated using an MGI DNBSEQ-T1 sequencer. ..

    Article Title: Divergent stem cell mechanisms govern the primary body axis and appendage regeneration in the axolotl
    Article Snippet: .. Stereo-seq sequencing files were generated from MGI DNBSEQ-T1 sequencer. ..

    Article Title: A spatial transcriptomics comparison of the adult versus metamorphosed axolotl brain.
    Article Snippet: .. Raw sequencing data were generated using an MGI DNBSEQ-T1 sequencer. ..

    Generated:

    Article Title: A spatial transcriptomics comparison of the adult versus metamorphosed axolotl brain
    Article Snippet: .. Raw sequencing data were generated using an MGI DNBSEQ-T1 sequencer. ..

    Article Title: Divergent stem cell mechanisms govern the primary body axis and appendage regeneration in the axolotl
    Article Snippet: .. Stereo-seq sequencing files were generated from MGI DNBSEQ-T1 sequencer. ..

    Article Title: A spatial transcriptomics comparison of the adult versus metamorphosed axolotl brain.
    Article Snippet: .. Raw sequencing data were generated using an MGI DNBSEQ-T1 sequencer. ..



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